Culture of chondrocytes on an acellular matrix derived frem amniochorionic membrane

  • José Manuel Lucero Fundación Biotar. Banco de Tejidos.
  • Alejandro Enrique Castiglioni Fundación Biotar. Banco de Tejidos.
  • Paula Hovanyecz Fundación Biotar. Banco de Tejidos.
  • R. M. Adrián Gorla Fuandación Biotar. Banco de Tejidos.
  • Oscar Berasategui Fundación Biotar. Banco de Tejidos.
  • Griselda Valeria Fedrigo Fundación Biotar. Banco de Tejidos.
  • Alicia Susana Lorenti Fundación Biotar. Banco de Tejidos.
DOI: https://doi.org/10.15417/104
Keywords: Amniochorionic membrane, Chondral lesions, Chondrocyte culture

Abstract

BackgroundHyaline cartilage has only a very restricted capability of regeneration in the adult. The incidence of chondral lesions at the knee is high, especially those of Grade II/III (Outerbridge). Therapies combining cells and biological scaffolds are promising biological approaches for the treatment of cartilage defects. The aim of this study is to analyze the characteristics of in vitro culture of human chondrocytes on decellularized amniochorionic membrane (ACM).MethodsBetween December 2010 and December 2011, 16 samples of cartilage from a living donor were processed, but only 7 of them were analyzed. Chondrocytes were grown and amplified on plastic and on ACM. The following analyses were carried out with those cells: interactions between cells and ACM; ACM capacity as a matrix for cells; and behavior of cells cultured on ACM.ResultsIn vitro chondrocytes exhibited phenotypic changes in the presence of ACM. The cells were able to adhere and remain on the spongy region of the membrane. Electron microscopy of cultured ACM showed cells, well preserved organelles, endoplasmic reticulum and desmosomes junctions.ConclusionsThe feasibility of culturing chondrocytes on ACM was shown in this work. The cells were able to adhere, remain and differentiate on this membrane during the study period.

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Author Biographies

José Manuel Lucero, Fundación Biotar. Banco de Tejidos.
Médico especialista en Ortopedia y Traumatología.Director Médico de Fundación Biotar. Banco de Tejidos. 
Alejandro Enrique Castiglioni, Fundación Biotar. Banco de Tejidos.
Médico Especialista en Ortopedia y Traumatología.Médico de Staff del Sanatorio Británico de Rosario. Sector Tumores Musculoesqueléticos.Coordinador médico. Fundación Biotar. Banco de Tejidos. 
Paula Hovanyecz, Fundación Biotar. Banco de Tejidos.
Estudiante avanzado de biotecnología.
R. M. Adrián Gorla, Fuandación Biotar. Banco de Tejidos.
Médico especialista en Medicina Transfusional.
Oscar Berasategui, Fundación Biotar. Banco de Tejidos.
Médico especialista en Ortopedia y Traumatología.
Griselda Valeria Fedrigo, Fundación Biotar. Banco de Tejidos.
Doctora en Biotecnología.
Alicia Susana Lorenti, Fundación Biotar. Banco de Tejidos.
Licenciada en química.Doctora de la UBA. Facultad de Medicina.Tissue Bank Specialist (AATB).

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Published
2012-12-11
How to Cite
Lucero, J. M., Castiglioni, A. E., Hovanyecz, P., Gorla, R. M. A., Berasategui, O., Fedrigo, G. V., & Lorenti, A. S. (2012). Culture of chondrocytes on an acellular matrix derived frem amniochorionic membrane. Revista De La Asociación Argentina De Ortopedia Y Traumatología, 77(3), 207-212. https://doi.org/10.15417/104
Issue
Vol 77 No 3 (2012)
Section
Basic Research

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