Alendronates administered at the fracture site. Experimental study in rabbits.
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Abstract
IntroductionThe aim of this experimental study was to evaluate the radiologic and histological results of the use of alendronate administered locally at the fractures site in rabbits.MethodsThe fractured right femur of 30 rabbits was used for evaluation. The animals were distributed in three groups of 10 rabbits each. A solution with alendronate was placed at the fracture site in group; the same procedure was performed 7 days after the fracture in group 2, and groiup 3 functioned as control. Radiographic evaluation was performed at the time of the fracture and at day 42. Radiological, PA and CT-scan evaluations of bone callus characteristics in each rabbit was performed.ResultsTwenty-four rabbits were evaluated (2 rabbits in group 2 and 4 in group 3 died). Histological evaluation evidenced moderated bone formation in the three groups without statistically significant differences (P=0,8336). Concerning imaging studies , there were no statistically significant differences in the size of bone callus among groups for both studies (X-rays: p=0,777 and CT: p=0,349).ConclusionThe use of alendronate administered locally at the fracture site, in the acute period and after one week, did not not alter the normal consolidation process determined by PA and radiology, six weeks after femur fracture in rabbits.
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Nuñez, J. E., Ruchelli, L. S., Bartolomeo, C., Flores, J. O., Iglesias, S. L., & Allende Nores, C. A. (2017). Alendronates administered at the fracture site. Experimental study in rabbits. Revista De La Asociación Argentina De Ortopedia Y Traumatología, 82(4), 327-333. https://doi.org/10.15417/699
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Basic Research
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References
1-Mochida Y, Bauer T, Akisue T, Brown PR. Alendronate does not inhibit early bone apposition to hydroxyapatite-coated total joint implants. A preliminary study. J Bone Joint Surg 2002; 84-A(2): 226-35.
2-.Fleisch H. Bisphosphonates: mechanism of action. Endocr Rev 1998; 19: 80-100.
3- Cruess, R.L., Dumont, J. Basic fracture healing. En: Newton, C.D., Nunamaker,
D.M.:Texbook of small animal orthopaedic. Ed. Lippincotr. Philadelphia, 1985.
4-Munns CFJ, Rauch F, Zeitlin L, Fassier F, Glorieux FH. Delayed osteotomy but not fracture healing in pediatric osteogenesis imperfecta patients receiving pamidronate. J Bone Miner Res 2004; 19(11): 1779-86.
5-Adolphson P, Abbaszadegan H, Bodén H, Salemyr M, Henriques T. Clodronate increases mineralization of callus after Colles’ fracture. A randomized, double-blind, placebo-controlled, prospective trial in 32 patients. Acta Orthop Scand 2000; 71(2); 195-200.
6-Ruchelli L, Allende C,Bruno P,Gutiérrez N,Flores O,Fernández Savoy. Integración de los Aloinjertos Óseos Impregnados con Alendronato: Estudio Experimental. Rev. Asoc. Argent. Ortop. Traumatol. 2014; 79 (4): 243-249
7- Li J, Mori S, Kaji Y, Mashiba T, Kawanishi J, Norimatsu H. Effect of bisphosphonate (incadronate) on fracture healing of bones in rats. J Bone Miner Res 1999; 14(6): 969-79
8-Li C, Mori S, Li J, Kaji Y, Akiyama T, Kawanishi J, Norimatsu H. Long-term effect of incadronate disodium (Ym-175) on fracture healing of femoral shaft in growing rats. J Bone Miner Res 2001; 16(3): 429-36.
9-Linde F, Sorenesen HC. The effect of different storage methods on the mechanical properties of trabecular bone. J Biomech 1993; 26(10): 1249-52.
10-Hyvönen PM, Karhi T, Kosma VM, Liimola-Luoma L, Hanijärvi H. The influence of dichloromethylene bisphosphonate on the healing of a long bone fracture, composition of bone mineral and histology of bone in the rat. Pharmacol Toxicol 1994; 75: 384-90.
11-Koivukangas A, Tuukkanen J, KippoK, Jamsa T, Hannuniemi R, Vaananen K, Jalovaara P. Long-term administration of clodronate does not prevent fracture healing in rats. Clin Orthop 2003; 408: 268-78.
12-Madsen JE, Berg-Larsen T, Kirkeby OJ, Falcch JA, Nordsletten L. No adverse effects of clodronate on fracture healing in rats. Acta Orthop Scand 1998; 69(5): 532-6.
13-Nyman MT, Paavolainen P, Lindhom TS. Clodronate increases the calcium content in fracture callus. An experimental study in rats. Arch Orthop Trauma Surg 1993; 112: 228 31.
14-Cao Y, Mori S, Mashiba T, Westmore MS, Ma L, Sato M, Akiyama T, Komatsubara S, Miyamoto K, Norimatsu H. Raloxifene, estrogen, and alendronate affect the processes of repair differently in ovariectomized rats. J Bone Miner Res 2002; 17(12): 2237-46.
15-Chastagnier D, Barbier A, de Vernejoul MC, Geusens P, Lacheret F. Effect of two bisphosphonates (tiludronate and etidronate) on bone healing. J Bone Miner Res 1993; 8: S236.
16-Flora L, Hassing GS, Cloyd CG, Bevan JA, Parfitt AM, Villanueva AR.The long term skeletal effects of EHDP in dogs. Metab Bone Dis Rel Res 1981; 3: 289-300.
17-Bauss F, Schenk RK, Hört S, Müller-Beckmann B, Sponer G. New model for simulation of fracture repair in full-grown beagle dogs: model characterization and results from a long-term study with ibandronate. J Pharmacol Toxicol Methods 2004; 50: 25-34.
18-Peter CP, Cook WO, Nunamaker DM, Provost MT, Seedor JG, Rodan GA. Effect of alendronate on fracture healing and bone remodeling in dogs. J Orthop Res 1996; 14(1): 74-9.
19-Adolphson P, Abbaszadegan H, Bodén H, Salemyr M, Henriques T.Clodronate increases mineralization of callus after Colles’ fracture. Arandomized, double-blind, placebo-controlled, prospective trial in 32 patients. Acta Orthop Scand 2000; 71(2); 195-200.
20-Van der Poest Clement E, Patka P, Vandormael K, Haarman H,Lips P. The effect of alendronate on bone mass after distal forearmfracture. J Bone Miner Res 2000; 15: 586-93.
21- Van der Poest Clement E, Van England M, Ader H, Roos JC, Patka P, Lips P. Alendronate in the prevention of bone loss after afracture of the lower leg. J Bone Miner Res 2002; 17: 2247-55.
22- Chastagnier D, Barbier A, de Vernejoul MC, Geusens P, Lacheret F. Effect of two bisphosphonates (tiludronate and etidronate) on bone healing. J Bone Miner Res 1993; 8: S236.
23-Flora L, Hassing GS, Cloyd CG, Bevan JA, Parfitt AM, Villanueva AR. The long term skeletal effects of EHDP in dogs. Metab Bone Dis Rel Res 1981; 3: 289-300.
24-Lenehan TM, Balligand M, Nunamaker DM, Wood FEJr. Effect of EHDP on fracture healing in dogs. J Orthop Res 1985; 3: 499-507.
25-Goodship AE, Walker PC, McNally D, Chambers T, Green JR1994 Use of a bisphosphonate (pamidronate) to modulate fracture repair in ovine bone. Ann Oncol 5:S53–S55.
26- Rozental TD, Makhni EC, Day CS, Bouxsein ML. Improving evaluation and treatment for osteoporosis following distal radial fractures. J Bone Joint Surg Am 2008; 90: 953-61.
27-Li J, Mori S, Kaji Y, Mashiba T, Kawanishi J, Norimatsu H. Effect of bisphosphonate (incadronate) on fracture healing of bones in rats. J Bone Miner Res 1999; 14(6): 969-79.
28- Li J, Mori S, Kaji Y, Kawanishi J, Akiyama T, Norimatsu H. Concentration of biphosphonate (incadronate) in callus areas and effects on fracture healing in rats. J Bone Miner Res 2000; 15(10):2042-51.
29- Li C, Mori S, Li J, Kaji Y, Akiyama T, Kawanishi J, Norimatsu H. Long-term effect of incadronate disodium (Ym-175) on fracture healing of femoral shaft in growing rats. J Bone Miner Res 2001; 16(3): 429-36.
30-Peter CP, Cook WO, Nunamaker DM, Provost MT, Seedor JG,Rodan GA. Effect of alendronate on fracture healing and bone remodeling in dogs. J Orthop Res 1996; 14(1): 74-9.
2-.Fleisch H. Bisphosphonates: mechanism of action. Endocr Rev 1998; 19: 80-100.
3- Cruess, R.L., Dumont, J. Basic fracture healing. En: Newton, C.D., Nunamaker,
D.M.:Texbook of small animal orthopaedic. Ed. Lippincotr. Philadelphia, 1985.
4-Munns CFJ, Rauch F, Zeitlin L, Fassier F, Glorieux FH. Delayed osteotomy but not fracture healing in pediatric osteogenesis imperfecta patients receiving pamidronate. J Bone Miner Res 2004; 19(11): 1779-86.
5-Adolphson P, Abbaszadegan H, Bodén H, Salemyr M, Henriques T. Clodronate increases mineralization of callus after Colles’ fracture. A randomized, double-blind, placebo-controlled, prospective trial in 32 patients. Acta Orthop Scand 2000; 71(2); 195-200.
6-Ruchelli L, Allende C,Bruno P,Gutiérrez N,Flores O,Fernández Savoy. Integración de los Aloinjertos Óseos Impregnados con Alendronato: Estudio Experimental. Rev. Asoc. Argent. Ortop. Traumatol. 2014; 79 (4): 243-249
7- Li J, Mori S, Kaji Y, Mashiba T, Kawanishi J, Norimatsu H. Effect of bisphosphonate (incadronate) on fracture healing of bones in rats. J Bone Miner Res 1999; 14(6): 969-79
8-Li C, Mori S, Li J, Kaji Y, Akiyama T, Kawanishi J, Norimatsu H. Long-term effect of incadronate disodium (Ym-175) on fracture healing of femoral shaft in growing rats. J Bone Miner Res 2001; 16(3): 429-36.
9-Linde F, Sorenesen HC. The effect of different storage methods on the mechanical properties of trabecular bone. J Biomech 1993; 26(10): 1249-52.
10-Hyvönen PM, Karhi T, Kosma VM, Liimola-Luoma L, Hanijärvi H. The influence of dichloromethylene bisphosphonate on the healing of a long bone fracture, composition of bone mineral and histology of bone in the rat. Pharmacol Toxicol 1994; 75: 384-90.
11-Koivukangas A, Tuukkanen J, KippoK, Jamsa T, Hannuniemi R, Vaananen K, Jalovaara P. Long-term administration of clodronate does not prevent fracture healing in rats. Clin Orthop 2003; 408: 268-78.
12-Madsen JE, Berg-Larsen T, Kirkeby OJ, Falcch JA, Nordsletten L. No adverse effects of clodronate on fracture healing in rats. Acta Orthop Scand 1998; 69(5): 532-6.
13-Nyman MT, Paavolainen P, Lindhom TS. Clodronate increases the calcium content in fracture callus. An experimental study in rats. Arch Orthop Trauma Surg 1993; 112: 228 31.
14-Cao Y, Mori S, Mashiba T, Westmore MS, Ma L, Sato M, Akiyama T, Komatsubara S, Miyamoto K, Norimatsu H. Raloxifene, estrogen, and alendronate affect the processes of repair differently in ovariectomized rats. J Bone Miner Res 2002; 17(12): 2237-46.
15-Chastagnier D, Barbier A, de Vernejoul MC, Geusens P, Lacheret F. Effect of two bisphosphonates (tiludronate and etidronate) on bone healing. J Bone Miner Res 1993; 8: S236.
16-Flora L, Hassing GS, Cloyd CG, Bevan JA, Parfitt AM, Villanueva AR.The long term skeletal effects of EHDP in dogs. Metab Bone Dis Rel Res 1981; 3: 289-300.
17-Bauss F, Schenk RK, Hört S, Müller-Beckmann B, Sponer G. New model for simulation of fracture repair in full-grown beagle dogs: model characterization and results from a long-term study with ibandronate. J Pharmacol Toxicol Methods 2004; 50: 25-34.
18-Peter CP, Cook WO, Nunamaker DM, Provost MT, Seedor JG, Rodan GA. Effect of alendronate on fracture healing and bone remodeling in dogs. J Orthop Res 1996; 14(1): 74-9.
19-Adolphson P, Abbaszadegan H, Bodén H, Salemyr M, Henriques T.Clodronate increases mineralization of callus after Colles’ fracture. Arandomized, double-blind, placebo-controlled, prospective trial in 32 patients. Acta Orthop Scand 2000; 71(2); 195-200.
20-Van der Poest Clement E, Patka P, Vandormael K, Haarman H,Lips P. The effect of alendronate on bone mass after distal forearmfracture. J Bone Miner Res 2000; 15: 586-93.
21- Van der Poest Clement E, Van England M, Ader H, Roos JC, Patka P, Lips P. Alendronate in the prevention of bone loss after afracture of the lower leg. J Bone Miner Res 2002; 17: 2247-55.
22- Chastagnier D, Barbier A, de Vernejoul MC, Geusens P, Lacheret F. Effect of two bisphosphonates (tiludronate and etidronate) on bone healing. J Bone Miner Res 1993; 8: S236.
23-Flora L, Hassing GS, Cloyd CG, Bevan JA, Parfitt AM, Villanueva AR. The long term skeletal effects of EHDP in dogs. Metab Bone Dis Rel Res 1981; 3: 289-300.
24-Lenehan TM, Balligand M, Nunamaker DM, Wood FEJr. Effect of EHDP on fracture healing in dogs. J Orthop Res 1985; 3: 499-507.
25-Goodship AE, Walker PC, McNally D, Chambers T, Green JR1994 Use of a bisphosphonate (pamidronate) to modulate fracture repair in ovine bone. Ann Oncol 5:S53–S55.
26- Rozental TD, Makhni EC, Day CS, Bouxsein ML. Improving evaluation and treatment for osteoporosis following distal radial fractures. J Bone Joint Surg Am 2008; 90: 953-61.
27-Li J, Mori S, Kaji Y, Mashiba T, Kawanishi J, Norimatsu H. Effect of bisphosphonate (incadronate) on fracture healing of bones in rats. J Bone Miner Res 1999; 14(6): 969-79.
28- Li J, Mori S, Kaji Y, Kawanishi J, Akiyama T, Norimatsu H. Concentration of biphosphonate (incadronate) in callus areas and effects on fracture healing in rats. J Bone Miner Res 2000; 15(10):2042-51.
29- Li C, Mori S, Li J, Kaji Y, Akiyama T, Kawanishi J, Norimatsu H. Long-term effect of incadronate disodium (Ym-175) on fracture healing of femoral shaft in growing rats. J Bone Miner Res 2001; 16(3): 429-36.
30-Peter CP, Cook WO, Nunamaker DM, Provost MT, Seedor JG,Rodan GA. Effect of alendronate on fracture healing and bone remodeling in dogs. J Orthop Res 1996; 14(1): 74-9.