Experimental Hypothermia Murine Model for Spinal Cord Injury Studies

  • Aníbal José Sarotto Laboratory of Experimental Neuropathology, Institute of Cell Biology and Neuroscience “Prof. E. De Robertis” (IBCN), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina https://orcid.org/0000-0002-2199-5524
  • Daniela Contartese Laboratory of Experimental Neuropathology, Institute of Cell Biology and Neuroscience “Prof. E. De Robertis” (IBCN), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina https://orcid.org/0000-0003-3690-264X
  • Verónica Dorfman Center for Biomedical, Biotechnological, Environmental and Diagnostic Studies (CEBBAD), Universidad Maimónides, Buenos Aires, Argentina https://orcid.org/0000-0002-7950-1400
  • Agustina Toscanini Instituto Nanobiotec (UBA-Conicet), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina https://orcid.org/0000-0001-9431-7794
  • Nicolás Scheverin Laboratory of Experimental Neuropathology, Institute of Cell Biology and Neuroscience “Prof. E. De Robertis” (IBCN), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina https://orcid.org/0000-0001-9878-0372
  • Micaela Besse Spinal Pathology Unit, Orthopedics and Traumatology Service, General Acute Care Hospital “Carlos G. Durand”, Autonomous City of Buenos Aires, Argentina https://orcid.org/0000-0002-4388-1384
  • Ignacio Larráyoz Angiogenesis Study Group, Center for Biomedical Research of La Rioja (CIBIR), Logroño, Spain https://orcid.org/0000-0003-1629-152X
  • Manuel Rey-Funes Laboratory of Experimental Neuropathology, Institute of Cell Biology and Neuroscience “Prof. E. De Robertis” (IBCN), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina https://orcid.org/0000-0002-0213-3056
  • Alfredo Martínez Angiogenesis Study Group, Center for Biomedical Research of La Rioja (CIBIR), Logroño, Spain https://orcid.org/0000-0003-4882-4044
  • César Fabián Loidl Laboratory of Experimental Neuropathology, Institute of Cell Biology and Neuroscience “Prof. E. De Robertis” (IBCN), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina https://orcid.org/0000-0001-6609-8969
DOI: https://doi.org/10.15417/issn.1852-7434.2022.87.1.1420
Keywords: Hypothermia, murines, spinal cord

Abstract

Introduction: Given the complexity of hypothermal trial systems in murines, they are expensive. Our objective was to evaluate if the exogenous hypothermal model used in our laboratory for ocular hypothermia was useful for a significant reduction in medullar spine temperature in adult murines. Materials and Methods: 36 60-day-old adult male Sprague-Dawley rats were used. They were separated into two groups: a normal temperature group at 24 °C (n=18) and a hypothermia group in a cold chamber at 8 °C for 180 minutes (n=18). Results: The mean rectal temperature was 37.71 ºC ± 0.572 in the normothermia group and 34.03ºC ± 0.250 in the hypothermia group (p <0.0001). The mean medullar temperature was 38.8 ± 0.468 ºC in the normothermia group and 36.4 ± 0.290 ºC in the hypothermia group (p <0.0001). Conclusion: Using systematic hypothermia in lab rats seems to be promising to evaluate physiologic and pathological mechanisms triggered in the medullar spine. Exposure to cold in the external chamber producessignificant medullar hypothermia in adult rats. Results suggest this might be an adequate and inexpensive medullar hypothermal model.

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Author Biographies

Aníbal José Sarotto, Laboratory of Experimental Neuropathology, Institute of Cell Biology and Neuroscience “Prof. E. De Robertis” (IBCN), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina
Laboratory of Experimental Neuropathology, Institute of Cell Biology and Neuroscience “Prof. E. De Robertis” (IBCN), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina
Daniela Contartese, Laboratory of Experimental Neuropathology, Institute of Cell Biology and Neuroscience “Prof. E. De Robertis” (IBCN), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina
Laboratory of Experimental Neuropathology, Institute of Cell Biology and Neuroscience “Prof. E. De Robertis” (IBCN), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina
Verónica Dorfman, Center for Biomedical, Biotechnological, Environmental and Diagnostic Studies (CEBBAD), Universidad Maimónides, Buenos Aires, Argentina
Center for Biomedical, Biotechnological, Environmental and Diagnostic Studies (CEBBAD), Universidad Maimónides, Buenos Aires, Argentina
Agustina Toscanini, Instituto Nanobiotec (UBA-Conicet), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina
Instituto Nanobiotec (UBA-Conicet), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina
Nicolás Scheverin, Laboratory of Experimental Neuropathology, Institute of Cell Biology and Neuroscience “Prof. E. De Robertis” (IBCN), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina
Laboratory of Experimental Neuropathology, Institute of Cell Biology and Neuroscience “Prof. E. De Robertis” (IBCN), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina
Micaela Besse, Spinal Pathology Unit, Orthopedics and Traumatology Service, General Acute Care Hospital “Carlos G. Durand”, Autonomous City of Buenos Aires, Argentina
Spinal Pathology Unit, Orthopedics and Traumatology Service, General Acute Care Hospital “Carlos G. Durand”, Autonomous City of Buenos Aires, Argentina
Ignacio Larráyoz, Angiogenesis Study Group, Center for Biomedical Research of La Rioja (CIBIR), Logroño, Spain
Angiogenesis Study Group, Center for Biomedical Research of La Rioja (CIBIR), Logroño, Spain
Manuel Rey-Funes, Laboratory of Experimental Neuropathology, Institute of Cell Biology and Neuroscience “Prof. E. De Robertis” (IBCN), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina
Laboratory of Experimental Neuropathology, Institute of Cell Biology and Neuroscience “Prof. E. De Robertis” (IBCN), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina
Alfredo Martínez, Angiogenesis Study Group, Center for Biomedical Research of La Rioja (CIBIR), Logroño, Spain
Angiogenesis Study Group, Center for Biomedical Research of La Rioja (CIBIR), Logroño, Spain
César Fabián Loidl, Laboratory of Experimental Neuropathology, Institute of Cell Biology and Neuroscience “Prof. E. De Robertis” (IBCN), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina
Laboratory of Experimental Neuropathology, Institute of Cell Biology and Neuroscience “Prof. E. De Robertis” (IBCN), School of Medicine, Universidad de Buenos Aires, CONICET, Autonomous City of Buenos Aires, Argentina

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Published
2022-02-14
How to Cite
Sarotto, A. J., Contartese, D., Dorfman, V., Toscanini, A., Scheverin, N., Besse, M., Larráyoz, I., Rey-Funes, M., Martínez, A., & Loidl, C. F. (2022). Experimental Hypothermia Murine Model for Spinal Cord Injury Studies. Revista De La Asociación Argentina De Ortopedia Y Traumatología, 87(1), 89-94. https://doi.org/10.15417/issn.1852-7434.2022.87.1.1420
Issue
Vol 87 No 1 (2022)
Section
Basic Research
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